In which phase do chromosomes align in the center of the cell during mitosis?

During mitosis, chromosomes align in the center of the cell during metaphase. This is a critical stage where the spindle fibers attach to the centromeres of the chromosomes, ensuring that each sister chromatid is properly aligned and prepared to be separated. The alignment in the middle of the cell, also referred to as the metaphase plate, is essential for the equal distribution of genetic material to the daughter cells. This precise arrangement facilitates an organized separation in the subsequent phase, ensuring that each new cell will receive an identical set of chromosomes. Understanding this alignment process highlights the importance of metaphase in the overall progression and accuracy of mitosis.